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Engineering the Activity of a Template-Independent DNA Polymerase.

ACS synthetic biology - 16 Aug 2024

Milisavljevic Marija, Rodriguez Teresa Rojas, Carlson Courtney K, Liu Chang C, Tyo Keith E J

Abstract excerpt

Enzymatic DNA writing technologies based on the template-independent DNA polymerase terminal deoxynucleotidyl transferase (TdT) have the potential to advance DNA information storage. TdT is unique in its ability to synthesize single-stranded DNA de novo but has limitations, including catalytic inhibition by ribonucleotide presence and slower incorporation rates compared to replicative polymerases. We anticipate...

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Deoxyribonucleotides
DNA-Directed DNA Polymerase
DNA Nucleotidylexotransferase
DNA, Single-Stranded
High-Throughput Screening Assays

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Engineering

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PMID: 39083642
DOI: 10.1021/acssynbio.4c00255

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