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Article

Impact of single-residue mutations on protein thermal stability: The case of threonine 83 of BC2L-CN lectin.

International journal of biological macromolecules - 1 Jun 2024

Hoya Megumi, Matsunaga Ryo, Nagatoishi Satoru, Ide Teruhiko, Kuroda Daisuke, Tsumoto Kouhei

Abstract excerpt

The thermal stability of trimeric lectin BC2L-CN was investigated and found to be considerably altered when mutating residue 83, originally a threonine, located at the fucose-binding loop. Mutants were analyzed using differential scanning calorimetry and isothermal microcalorimetry. Although most mutations decreased the affinity of the protein for oligosaccharide H type 1, six mutations increased the melting...

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PMID: 38815947
DOI: 10.1016/j.ijbiomac.2024.132682

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