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Article

Fluorescent primers amplification refractory mutation system qPCR (FP ARMS-qPCR) for MTHFR C677T SNP genotyping.

Molecular biology reports - 6 Nov 2024

Han Yongjun, Wu Hong

Abstract excerpt

BACKGROUND: The currently used methods for the detection of methylene tetrahydrofolic acid reductase (MTHFR) C677T single nucleotide polymorphism (SNP) are either time-consuming or expensive. In this study, we devised an accurate, rapid and easy-to-use SNP detection system based on fluorescent primers amplification refractory mutation system qPCR, known as FP ARMS-qPCR. METHODS: Fluorescent primers (FPs) modified...

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PMID: 39503982
DOI: 10.1007/s11033-024-10036-3

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