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Article

Genome editing of a recalcitrant wine grape genotype by lipofectamine-mediated delivery of CRISPR/Cas9 ribonucleoproteins to protoplasts.

The Plant journal : for cell and molecular biology - 1 Jul 2024

Gambino Giorgio, Nuzzo Floriana, Moine Amedeo, Chitarra Walter, Pagliarani Chiara, Petrelli Annalisa, Boccacci Paolo, Delliri Andrea, Velasco Riccardo, Nerva Luca, Perrone Irene

Abstract excerpt

The main bottleneck in the application of biotechnological breeding methods to woody species is due to the in vitro regeneration recalcitrance shown by several genotypes. On the other side, woody species, especially grapevine (Vitis vinifera L.), use most of the pesticides and other expensive inputs in agriculture, making the development of efficient approaches of genetic improvement absolutely urgent. Genome...

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PMID: 38646817
DOI: 10.1111/tpj.16770

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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