Article
Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon.
Scientific reports - 19 Nov 2019
Hooghvorst Isidre, López-Cristoffanini Camilo, Nogués Salvador
Abstract excerpt
CRISPR/Cas9 system has been widely applied in many plant species to induce mutations in the genome for studying gene function and improving crops. However, to our knowledge, there is no report of CRISPR/Cas9-mediated genome editing in melon (Cucumis melo). In our study, phytoene desaturase gene of melon (CmPDS) was selected as target for the CRISPR/Cas9 system with two designed gRNAs, targeting exons 1 and 2. A...
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