Article
Protoplast-Based Method for Genome Editing in Tetraploid Potato.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Nicolia Alessandro, Fält Ann-Sofie, Hofvander Per, Andersson Mariette
Abstract excerpt
The cultivated potato is tetraploid with four probably equivalent loci for each gene. A potato variety is furthermore commonly genetically heterogeneous and selected based on a beneficial genetic context which is maintained by clonal propagation. When introducing genetic changes by genome editing it is then desirable to achieve edits in all four loci for a certain gene target. This is in order to avoid crosses to...
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