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Article

Construction of a New Agrobacterium tumefaciens-Mediated Transformation System based on a Dual Auxotrophic Approach in Cordyceps militaris.

Journal of microbiology and biotechnology - 28 May 2024

Yan Huan Huan, Shang Yi Tong, Wang Li Hong, Tian Xue Qin, Tran Van-Tuan, Yao Li Hua, Zeng Bin, Hu Zhi Hong

Abstract excerpt

Cordyceps militaris is a significant edible fungus that produces a variety of bioactive compounds. We have previously established a uridine/uracil auxotrophic mutant and a corresponding Agrobacterium tumefaciens-mediated transformation (ATMT) system for genetic characterization in C. militaris using pyrG as a screening marker. In this study, we constructed an ATMT system based on a dual pyrG and hisB auxotrophic...

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Identifiers

PMID: 38563100
DOI: 10.4014/jmb.2312.12003
PMCID: PMC11180907

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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