Article
Development of an activation tagging system for the basidiomycetous medicinal fungus Antrodia cinnamomea.
Mycological research - 1 Mar 2009
Chen Emily Chin-Fun, Su Yu-Hsiu, Kanagarajan Selvaraju, Agrawal Dinesh Chandra, Tsay Hsin-Sheng
Abstract excerpt
This study describes the development of an efficient and reliable activation tagging system for the medicinal fungus Antrodia cinnamomea. For successful Agrobacterium tumefaciens-mediated transformation, different parameters were considered. The Agrobacterium concentration of 5 x 10(8) cfu ml(-1), 1mm acetosyringone, 25-d-old mycelia at 0.2 g ml(-1), and co-culture period of 6d were found to be the most optimal...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
