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Efficient disruption of CmHk1 using CRISPR/Cas9 ribonucleoprotein delivery in Cordyceps militaris.

FEMS microbiology letters - 17 Jan 2023

Choi Hyeongju, Park Sung-Won, Oh Junsang, Kim Cheol Soo, Sung Gi-Ho, Sang Hyunkyu

Abstract excerpt

Cordyceps militaris, an entomopathogenic ascomycete, produces edible medicinal mushrooms known to have medicinal and therapeutic functions. To develop the genetic transformation system in C. militaris, green fluorescent protein (GFP) mutants of C. militaris were generated by PEG-mediated protoplast transformation. The CRISPR/Cas9 ribonucleoprotein (RNP) targeting the class III histidine kinase of C. militaris...

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PMID: 37475654
DOI: 10.1093/femsle/fnad072

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