Production and Discovery of Novel Recombinant Adeno‐Associated Viral Vectors

doi:10.1002/9780471729259.mc14d01s26

Article

Production and Discovery of Novel Recombinant Adeno‐Associated Viral Vectors

1 Aug 2012

Abstract excerpt

In this unit, we describe the detailed procedure for a three-plasmid transfection method for rAAV production, and discuss its advantages, limitations, and troubleshooting techniques. We further discuss the rAAV purification process using CsCl gradients, as well as subsequent quality control methods using SDS-PAGE and real-time PCR to assess vector purity, packaging efficiency, and viral titer. Finally, we...

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