Article
A method for isolating contractile smooth muscle cells from cryopreserved tissue.
American journal of physiology. Cell physiology - 1 Mar 2024
Schultz Matheus L C, Seth Pranjal, Kachmar Linda, Ijpma Gijs, Lauzon Anne-Marie
Abstract excerpt
Multiple techniques have been developed to isolate contractile smooth muscle cells (SMCs) from tissues with varying degrees of success. However, most of these approaches rely on obtaining fresh tissue, which poses logistical challenges. In the present study, we introduce a novel protocol for isolating contractile SMCs from cryopreserved smooth muscle (SM) tissue, thereby enhancing experimental efficiency. This...
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