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Article

Optimized method for isolating highly purified and functional porcine aortic endothelial and smooth muscle cells.

Journal of cellular physiology - 1 Nov 2017

Beigi Farideh, Patel Mitalben, Morales-Garza Marco A, Winebrenner Caitlin, Gobin Andrea S, Chau Eric, Sampaio Luiz C, Taylor Doris A

Abstract excerpt

Numerous protocols exist for isolating aortic endothelial and smooth muscle cells from small animals. However, establishing a protocol for isolating pure cell populations from large animal vessels that are more elastic has been challenging. We developed a simple sequential enzymatic approach to isolate highly purified populations of porcine aortic endothelial and smooth muscle cells. The lumen of a porcine aorta...

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Identifiers

PMID: 28059438
DOI: 10.1002/jcp.25764

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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