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Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators

1 Dec 2023

Abstract excerpt

Ferroptosis, an iron-dependent programmed cell death triggered by excessive lipid peroxidation, has shown promising therapeutic potentials in human diseases. Here, we describe a protocol of a CRISPR-Cas9 loss-of-function screen to identify regulators in response to different inducers of ferroptosis. We emphasize the steps of library amplification, drug treatment, high-throughput sequencing preparation, and...

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PMID: 38048220
DOI: 10.1016/j.xpro.2023.102762

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