Article

Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene

2022-03-25

Abstract excerpt

In humans, disruptions in the heme biosynthetic pathway are associated with various types of porphyrias, including variegate porphyria that results from the decreased activity of protoporphyrinogen oxidase IX (PPO; E.C.1.3.3.4), the enzyme catalyzing the penultimate step of the heme biosynthesis. Here we report the generation and characterization of human cell lines, in which PPO was inactivated using the CRISPR/C...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5213b27f-2888-5661-b01a-7981d54df892
DOI: 10.21203/rs.3.rs-1472782/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX geneDOI 10.21203/rs.3.rs-1472782/v1Read

Porphyrin overdrive rewires cancer cell metabolismSemantic proximity Read

Mutation-Specific Guide RNA for Compound Heterozygous Porphyria On-target Scarless Correction by CRISPR/Cas9 in Stem Cells.Semantic proximity Read

Porphyrin overdrive rewires pan-cancer cell metabolismSemantic proximity Read

Zebrafish models for human ALA-dehydratase-deficient porphyria (ADP) and hereditary coproporphyria (HCP) generated with TALEN and CRISPR-Cas9Semantic proximity Read

Acute hepatic and erythropoietic porphyrias: from ALA synthases 1 and 2 to new molecular bases and treatments.Semantic proximity Read

Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosisSemantic proximity Read

Reduced PPOX Expression Causes Intrinsic Pathogenicity in Keratinocytes, Contributing to the Cutaneous Variegate Porphyria Phenotype.Semantic proximity Read

The porphyrias: advances in diagnosis and treatmentSemantic proximity Read

The porphyrias: advances in diagnosis and treatment.Semantic proximity Read

Generation and characterization of CRISPR-Cas9-Mediated <i>XPC</i> Gene Knockout in Human Skin CellsSemantic proximity Read

Updates on the diagnosis and management of the most common hereditary porphyrias: AIP and EPP.Semantic proximity Read