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Article

Evaluating the accuracy of amplicon-based microbiome computational pipelines on simulated human gut microbial communities

30 May 2017

Abstract excerpt

BACKGROUND: Microbiome studies commonly use 16S rRNA gene amplicon sequencing to characterize microbial communities. Errors introduced at multiple steps in this process can affect the interpretation of the data. Here we evaluate the accuracy of operational taxonomic unit (OTU) generation, taxonomic classification, alpha- and beta-diversity measures for different settings in QIIME, MOTHUR and a pplacer-based...

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PMID: 28558684
DOI: 10.1186/s12859-017-1690-0

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