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Article

A method for TAT-Cre recombinase-mediated floxed allele modification in ex vivo tissue slices.

Disease models & mechanisms - 1 Nov 2023

Cheong Sek-Shir, Luis Tiago C, Stewart Michelle, Hillier Rosie, Hind Matthew, Dean Charlotte H

Abstract excerpt

Precision-cut lung slices (PCLS) are used for a variety of applications. However, methods to manipulate genes in PCLS are currently limited. We developed a new method, TAT-Cre recombinase-mediated floxed allele modification in tissue slices (TReATS), to induce highly effective and temporally controlled gene deletion or activation in ex vivo PCLS. Treatment of PCLS from Rosa26-flox-stop-flox-EYFP mice with...

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PMID: 37828896
DOI: 10.1242/dmm.050267
PMCID: PMC10629676

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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