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Article

Allele-specific quantification of human leukocyte antigen transcript isoforms by nanopore sequencing.

Frontiers in immunology - 1 Jan 2023

Hughes Andrew E O, Montgomery Maureen C, Liu Chang, Weimer Eric T

Abstract excerpt

Introduction: While tens of thousands of HLA alleles have been identified by DNA sequencing, the contribution of alternative splicing to HLA diversity is not well characterized. In this study, we sought to determine if long-read sequencing could be used to accurately quantify allele-specific HLA transcripts in primary human lymphocytes. Methods: cDNA libraries were prepared from peripheral blood lymphocytes from...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 37662944
DOI: 10.3389/fimmu.2023.1199618
PMCID: PMC10471969

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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