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Development of a POCT detection platform based on a locked nucleic acid-enhanced ARMS-RPA-GoldMag lateral flow assay.

Journal of pharmaceutical and biomedical analysis - 25 Oct 2023

Liu Yaqun, Huang Huiying, Zheng Yuzhong, Wang Chunfang, Chen Wencheng, Huang Weiyi, Lin Liyun, Wei Huagui, Wang Junli, Lin Min

Abstract excerpt

In this study, a novel genotyping point-of-care testing (POCT) rapid detection device, the locked nucleic acid (LNA)-amplification refractory mutation system (ARMS)-recombinase polymerase amplification (RPA)-GoldMag lateral flow assay (LFA) platform, was provided by mining and synthesis based on prior technology. Research methods based on system-integrated innovation and knowledge-integrated generation have...

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Gold
Metal Nanoparticles
Mutation
Recombinases
Sensitivity and Specificity

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PMID: 37573622
DOI: 10.1016/j.jpba.2023.115632

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