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Misexpression Approaches for the Manipulation of Flower Development.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Xu Yifeng, Gan Eng-Seng, Ito Toshiro

Abstract excerpt

The generation of dominant gain-of-function mutants through activation tagging is a forward genetic approach that can be applied to study the mechanisms of flower development, complementing the screening of loss-of-function mutants. In addition, the functions of genes of interest can be further analyzed through reverse genetics. A commonly used method is gene overexpression, where ectopic expression can result in...

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PMID: 37540372
DOI: 10.1007/978-1-0716-3299-4_21

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