Article
Misexpression approaches for the manipulation of flower development.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Xu Yifeng, Gan Eng-Seng, Ito Toshiro
Abstract excerpt
The generation of dominant gain-of-function mutants through activation tagging is a forward genetic approach that complements the screening of loss-of-function mutants and that has been successfully applied to studying the mechanisms of flower development. In addition, the functions of genes of interest can be further analyzed through reverse genetics. A commonly used method is gene overexpression, where strong,...
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