Article
Identification of seed dormancy mutants by activation tagging.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Zhao Tiehan, Zeng Ying, Kermode Allison R
Abstract excerpt
Activation tagging is an important tool for gene discovery in plants. This method utilizes a T-DNA sequence that contains four tandem copies of the cauliflower mosaic virus 35S enhancer sequence or promoters oriented outward to the T-DNA border sequences. These elements enhance the expression of genes neighboring on either side of the randomly integrated T-DNA, resulting in gain-of-function phenotypes. Activation...
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