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RART-LAMP: One-Step Extraction-Free Method for Genotyping within 40 min.

Analytical chemistry - 22 Aug 2023

Zhang Zhang, Guan Luhao, Yao Juan, Li Lijia, Liu Chunfang, Guo Yongcan, Xie Guoming

Abstract excerpt

Loop-mediated isothermal amplification (LAMP) is a commonly used alternative to PCR for point-of-care detection of nucleic acids due to its rapidity, sensitivity, specificity, and simpler instrumentation. While dual-labeled TaqMan probes are widely used in PCR for single-nucleotide polymorphism (SNP) genotyping, real-time LAMP primarily relies on turbidimetry or intercalator fluorescence measurements, which can...

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PMID: 37534990
DOI: 10.1021/acs.analchem.3c02232

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