Article

A protocol for the detection of genetic markers in saliva by polymerase chain reaction without a nucleic acid purification step: examples of SARS-CoV-2 and GAPDH markers.

2021-04-08

Abstract excerpt

<title>Abstract</title> <p><bold>Background:</bold> The objective of this study was to develop a protocol for direct use of saliva in tests for genetic markers, without purification of nucleic acids. Currently, diagnostic tests use purified nucleic acids from clinical samples. This purification step adds time, cost, and affects the quality of testing. Multiple attempts to remove the purification step were reporte...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 25c5e97c-328f-598f-86a1-95b92fa2a359
DOI: 10.21203/rs.3.rs-375729/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A protocol for the detection of genetic markers in saliva by polymerase chain reaction without a nucleic acid purification step: examples of SARS-CoV-2 and GAPDH markers.DOI 10.21203/rs.3.rs-375729/v1Read

Wide application of minimally processed saliva on multiple RT-qPCR kits for SARS-CoV-2 detection in IndonesiaSemantic proximity Read

Extraction-free colorimetric RT-LAMP for SARS-CoV-2 RNA detection from salivaSemantic proximity Read

Saliva Sampling and Its Direct Lysis, an Excellent Option To Increase the Number of SARS-CoV-2 Diagnostic Tests in Settings with Supply ShortagesSemantic proximity Read

A clinically validated human saliva metatranscriptomic test for global systems biology studiesSemantic proximity Read

Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settingsSemantic proximity Read

Single-tube collection and nucleic acid analysis of clinical samples: a rapid approach for SARS-CoV-2 saliva testingSemantic proximity Read

Performance of saliva as a specimen to detect SARS-CoV-2Semantic proximity Read

Comparison Between a Standard and SalivaDirect RNA Extraction Protocol for Molecular Diagnosis of SARS-CoV-2 Using Nasopharyngeal Swab and Saliva Clinical SamplesSemantic proximity Read

Assessment of the use and quick preparation of saliva for rapid microbiological diagnosis of COVID-19Semantic proximity Read

Saliva as a Potential Clinical Specimen for Diagnosis of SARS-CoV-2Semantic proximity Read

Equivalence of saliva RT-qPCR testing to nasal-throat/nasopharyngeal swab testing in the general practitioner’s setting to detect SARS-CoV-2Semantic proximity Read

RT-PCR Screening Tests for SARS-CoV-2 with Saliva Samples in Asymptomatic People: Strategy to Maintain Social and Economic Activities while Reducing the Risk of Spreading the VirusSemantic proximity Read