Article
Visualized detection of single-base difference in multiplexed loop-mediated isothermal amplification amplicons by invasive reaction coupled with oligonucleotide probe-modified gold nanoparticles.
Biosensors & bioelectronics - 15 Apr 2017
Lu Yan, Ma Xueping, Wang Jianping, Sheng Nan, Dong Tianhui, Song Qinxin, Rui Jianzhong, Zou Bingjie, Zhou Guohua
Abstract excerpt
Loop-mediated isothermal amplification (LAMP) is a well-developed DNA amplification method with an ultra-high sensitivity, but it is difficult to recognize a single-base difference (like genotyping) in target-specific amplicons by conventional detection ways, such as the intercalation of dyes into dsDNA amplicons or the increase of solution turbidity along with the polymerization process. To allow genotyping...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.