Back to search

Article

Extraction-free colorimetric RT-LAMP for SARS-CoV-2 RNA detection from saliva

2024-08-09

Abstract excerpt

<h4>Aim</h4> To develop a rapid and easy diagnostic assay for detection of SARS-CoV-2 RNA presented in saliva samples. <h4>Method</h4> The color-based RT-LAMP was used to detect nucleocapsid (N) gene of SARS-CoV-2 without RNA extraction from saliva. <h4>Results</h4> RNA spiked saliva can be used directly for cDNA synthesis after heat inactivation of the saliva and diluted 2-fold with either water or PBS. For bo...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
2040c317-cef8-5378-8b0b-417cbb467c9c
DOI
10.1101/2024.08.09.607279
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Extraction-free colorimetric RT-LAMP for SARS-CoV-2 RNA detection from salivaDOI 10.1101/2024.08.09.607279
A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samplesSemantic proximity
Wide application of minimally processed saliva on multiple RT-qPCR kits for SARS-CoV-2 detection in IndonesiaSemantic proximity
Performance of saliva as a specimen to detect SARS-CoV-2Semantic proximity
Development of a Saliva-Optimized RT-LAMP Assay for SARS-CoV-2Semantic proximity
Developing RT-LAMP Assays for Detection of SARS-CoV-2 in SalivaSemantic proximity
Development of an Integrated Sample Amplification Control for Salivary Point-of-Care Pathogen TestingSemantic proximity
EasyCOV : LAMP based rapid detection of SARS-CoV-2 in salivaSemantic proximity
A protocol for the detection of genetic markers in saliva by polymerase chain reaction without a nucleic acid purification step: examples of SARS-CoV-2 and GAPDH markers.Semantic proximity
Assessment of the use and quick preparation of saliva for rapid microbiological diagnosis of COVID-19Semantic proximity
Equivalence of saliva RT-qPCR testing to nasal-throat/nasopharyngeal swab testing in the general practitioner’s setting to detect SARS-CoV-2Semantic proximity
A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samplesSemantic proximity
A Colorimetric RT-LAMP Detection of Multiple SARS-CoV-2 Variants of Concern and Lineages from Direct Nasopharyngeal Swab Samples without RNA IsolationSemantic proximity
Select a neighboring publication to make it the new centre.