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CRISPR-Cas13a-Based Assay for Accurate Detection of OXA-48 and GES Carbapenemases

19 Jul 2023

Abstract excerpt

Carbapenems are one of the last-resort antibiotics for defense against multidrug-resistant pathogens. Multiple nucleic acid amplification methods, including multiplex PCR, multiplex loop-mediated isothermal amplification (LAMP) and multiplex RPAs, can achieve rapid, accurate, and simultaneous detection of several resistance genes to carbapenems in a single reaction. However, these assays need thermal cycling...

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PMID: 37466441
DOI: 10.1128/spectrum.01329-23

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