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Detection of KPC-Producing Carbapenem-Resistant Klebsiella pneumoniae Based on CRISPR Cas12a

12 Jun 2025

Abstract excerpt

To develop a detection system for Klebsiella pneumoniae carbapenemase (KPC) and provide a reference for clinical prevention and control of nosocomial infections caused by multidrug-resistant K. pneumoniae.The KPC resistance gene was amplified by PCR.Guided by crRNA, Cas12a specifically identified the resistance gene and activated its trans-cleavage activity.In the detection system, a fluorescence probe was...

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PMID: 40537891
DOI: 10.4014/jmb.2502.02042

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