Scanning mutagenesis of the voltage-gated sodium channel NaV1.2 using base editing.

doi:10.1016/j.celrep.2023.112563

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Article

Scanning mutagenesis of the voltage-gated sodium channel NaV1.2 using base editing.

Cell reports - 27 Jun 2023

Pablo Juan Lorenzo B, Cornett Savannah L, Wang Lei A, Jo Sooyeon, Brünger Tobias, Budnik Nikita, Hegde Mudra, DeKeyser Jean-Marc, Thompson Christopher H, Doench John G, Lal Dennis, George Alfred L, Pan Jen Q

Abstract excerpt

It is challenging to apply traditional mutational scanning to voltage-gated sodium channels (NaVs) and functionally annotate the large number of coding variants in these genes. Using a cytosine base editor and a pooled viability assay, we screen a library of 368 guide RNAs (gRNAs) tiling NaV1.2 to identify more than 100 gRNAs that change NaV1.2 function. We sequence base edits made by a subset of these gRNAs to...

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PMID: 37267104
DOI: 10.1016/j.celrep.2023.112563
PMCID: PMC10592450

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