Article
Genome-wide CRISPR screening identifies cellular factors controlling nonviral genome editing efficiency.
Nature communications - 13 Aug 2026
Saxena Shivani, Kabra Meha, Abdeen Amr A, Tabima Diana M, Sinha Divya, Rawding Piper A, Zhu Min, Xie Ruosen, Kulkarni Tanvi, Hanstad Giovanni M, Fernandez Zepeda Maria A, Gamm David M, Pattnaik Bikash R, Gong Shaoqin, Saha Krishanu
Abstract excerpt
To systematically map cellular factors constraining nonviral genome editing, influencing uptake and intracellular trafficking, we develop a genome-wide CRISPR screening platform linking perturbation of 19,114 genes to editing outcomes in human cells. We identify six negative regulators of delivery whose depletion increases editing efficiency by up to six-fold across diverse payloads, loci, and cell types. We test...
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