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Single-Particle Tracking of Thermomyces lanuginosus Lipase Reveals How Mutations in the Lid Region Remodel Its Diffusion.

Biomolecules - 31 Mar 2023

Iversen Josephine F, Bohr Søren S-R, Pinholt Henrik D, Moses Matias E, Iversen Lars, Christensen Sune M, Hatzakis Nikos S, Zhang Min

Abstract excerpt

The function of most lipases is controlled by the lid, which undergoes conformational changes at a water-lipid interface to expose the active site, thus activating catalysis. Understanding how lid mutations affect lipases' function is important for designing improved variants. Lipases' function has been found to correlate with their diffusion on the substrate surface. Here, we used single-particle tracking (SPT),...

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PMID: 37189378
DOI: 10.3390/biom13040631
PMCID: PMC10135605

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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