Article
Novel Method for Detection of PIK3CA Mutations in Circulating Tumor DNA of Patients with Colorectal Cancer.
Applied biochemistry and biotechnology - 1 Dec 2023
Pu Wangyang, Wang Fengjiao, Li Kai, Xing Chungen, Zhuang Zhixiang, Wang Hui, Bian Huahui, Zhang Rong, Xiao Li
Abstract excerpt
The PIK3CA mutation is considered a potential target for treatment of colorectal cancer. We evaluated a PIK3CA mutation assay on plasma cell-free DNA (cfDNA) using a newly developed PCR with restriction digestion integrated and followed by Sanger's sequencing. We analyzed PIK3CA mutation in plasma with our newly developed assays and in matching tumor tissues by routine methods. We detected the PIK3CA gene...
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