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Article

ddPCR Overcomes the CRISPR-Cas13a-Based Technique for the Detection of the BRAF p.V600E Mutation in Liquid Biopsies.

International journal of molecular sciences - 10 Oct 2024

Palacín-Aliana Irina, García-Romero Noemí, Carrión-Navarro Josefa, Puig-Serra Pilar, Torres-Ruiz Raul, Rodríguez-Perales Sandra, Viñal David, González-Rumayor Víctor, Ayuso-Sacido Ángel

Abstract excerpt

The isolation of circulating tumoral DNA (ctDNA) present in the bloodstream brings about the opportunity to detect genomic aberrations from the tumor of origin. However, the low amounts of ctDNA present in liquid biopsy samples makes the development of highly sensitive techniques necessary to detect targetable mutations for the diagnosis, prognosis, and monitoring of cancer patients. Here, we employ standard...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39456686
DOI: 10.3390/ijms252010902
PMCID: PMC11507125

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