Article
Time-saving method for directly amplifying and capturing a minimal amount of pancreatic tumor-derived mutations from fine-needle aspirates using digital PCR.
Scientific reports - 23 Jul 2020
Ono Yusuke, Hayashi Akihiro, Maeda Chiho, Suzuki Mayumi, Wada Reona, Sato Hiroki, Kawabata Hidemasa, Okada Tetsuhiro, Goto Takuma, Karasaki Hidenori, Mizukami Yusuke, Okumura Toshikatsu
Abstract excerpt
It is challenging to secure a cytopathologic diagnosis using minute amounts of tumor fluids and tissue fragments. Hence, we developed a rapid, accurate, low-cost method for detecting tumor cell-derived DNA from limited amounts of specimens and samples with a low tumor cellularity, to detect KRAS mutations in pancreatic ductal carcinomas (PDA) using digital PCR (dPCR). The core invention is based on the suspension...
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