Article
Modified Allele-Specific qPCR (ASQ) Genotyping.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Amangeldiyeva Aigul, Baidyussen Akmaral, Kuzbakova Marzhan, Yerzhebayeva Raushan, Jatayev Satyvaldy, Shavrukov Yuri
Abstract excerpt
The allele-specific qPCR (ASQ) method for SNP (single nucleotide polymorphism) detection is based on the FRET (fluorescence resonance energy transfer) system, a system using position-dependent fluorescent dyes and quenches. The modified ASQ method requires two separate components: (1) the allele-specific part, two AS primers targeting the SNP with identity in the penultimate positions at the 3'-end and specific...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.