Article
An alternative genotyping method using dye-labeled universal primer to reduce unspecific amplifications.
Molecular biology reports - 1 Apr 2010
de Arruda Maurício Papa, Gonçalves Evonnildo Costa, Schneider Maria Paula Cruz, da Silva Artur Luiz da Costa, Morielle-Versute Eliana
Abstract excerpt
We proposed a modification the procedure of genotyping based in labeled universal primer and tailed primer. In the standard protocol, three primers are used in the same PCR reaction, a forward primer with tail added at the 5' end of the identical sequence to labeled universal primer with dye-fluorescent and a reverse primer. Unfortunately, the choice of a labeled primer characterized by a large number of...
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