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Article

A culture platform to study quiescent hematopoietic stem cells following genome editing

1 Dec 2022

Abstract excerpt

Other than genetically engineered mice, few reliable platforms are available for the study of hematopoietic stem cell (HSC) quiescence. Here we present a platform to analyze HSC cell cycle quiescence by combining culture conditions that maintain quiescence with a CRISPR-Cas9 genome editing system optimized for HSCs. We demonstrate that preculture of HSCs enhances editing efficiency by facilitating nuclear...

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PMID: 36590688
DOI: 10.1016/j.crmeth.2022.100354

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