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Article

Quantitative imaging of single-cell phenotypes in cancer cells cultured on hydrogel surfaces.

STAR protocols - 17 Mar 2023

Bousgouni Vicky, Bakal Chris

Abstract excerpt

Small interfering RNA (siRNA) screening approaches used with quantitative single-cell analysis can uncover the roles of genes in cell morphogenesis. Here, we present a high-throughput automated phenotypic screening technique to quantify a single cell shape in cancer cells cultured on top of soft 3D hydrogels. We describe reverse transfection of cells with siRNAs and seeding of these cells on top of collagen,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 36525347
DOI: 10.1016/j.xpro.2022.101942
PMCID: PMC9792547

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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