Article
Development of a system for genetic manipulation of Bartonella bacilliformis.
Applied and environmental microbiology - 1 Aug 1999
Battisti J M, Minnick M F
Abstract excerpt
Lack of a system for site-specific genetic manipulation has severely hindered studies on the molecular biology of all Bartonella species. We report the first site-specific mutagenesis and complementation for a Bartonella species. A highly transformable strain of B. bacilliformis, termed JB584, was isolated and found to exhibit a significant increase in transformation efficiency with the broad-host-range plasmid...
Topics
- Bacteriological Techniques
- Bartonella
- Base Sequence
- DNA Primers
- DNA, Bacterial
- Flagellin
- Genes, Bacterial
- Genetic Complementation Test
- Genetic Techniques
- Genetic Vectors
- Microscopy, Electron
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Hybridization
- Plasmids
- Polymerase Chain Reaction
- Transformation, Genetic