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Article

SpikeSeq: A rapid, cost efficient and simple method to identify SARS-CoV-2 variants of concern by Sanger sequencing part of the spike protein gene.

Journal of virological methods - 1 Feb 2023

Jørgensen Tue Sparholt, Pedersen Martin Schou, Blin Kai, Kuntke Franziska, Salling Henrik K, Marvig Rasmus L, Michaelsen Thomas Y, Albertsen Mads, Larsen Helene

Abstract excerpt

In 2020, the novel coronavirus, SARS-CoV-2, caused a pandemic, which is still raging at the time of writing this. Here, we present results from SpikeSeq, the first published Sanger sequencing-based method for the detection of Variants of Concern (VOC) and key mutations, using a 1 kb amplicon from the recognized ARTIC Network primers. The proposed setup relies entirely on materials and methods already in use in...

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Identifiers

PMID: 36368344
DOI: 10.1016/j.jviromet.2022.114648
PMCID: PMC9642040

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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