Article
Mapping Maize Mutants Using Bulked-Segregant Analysis and Next-Generation Sequencing.
Current protocols - 1 Nov 2022
Best Norman B, McSteen Paula
Abstract excerpt
Forward genetics is used to identify the genetic basis for a phenotype. The approach involves identifying a mutant organism exhibiting a phenotype of interest and then mapping the causative locus or gene. Bulked-segregant analysis (BSA) is a quick and effective approach to map mutants using pools of mutants and wild-type plants from a segregating population to identify linkage of the mutant phenotype, and this...
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