Article
High-throughput genetic mapping of mutants via quantitative single nucleotide polymorphism typing.
Genetics - 1 Jan 2010
Liu Sanzhen, Chen Hsin D, Makarevitch Irina, Shirmer Rebecca, Emrich Scott J, Dietrich Charles R, Barbazuk W Brad, Springer Nathan M, Schnable Patrick S
Abstract excerpt
Advances in next-generation sequencing technology have facilitated the discovery of single nucleotide polymorphisms (SNPs). Sequenom-based SNP-typing assays were developed for 1359 maize SNPs identified via comparative next-generation transcriptomic sequencing. Approximately 75% of these SNPs were successfully converted into genetic markers that can be scored reliably and used to generate a SNP-based genetic map...
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