Article
A Population of Deletion Mutants and an Integrated Mapping and Exome-seq Pipeline for Gene Discovery in Maize.
G3 (Bethesda, Md.) - 9 Aug 2016
Jia Shangang, Li Aixia, Morton Kyla, Avoles-Kianian Penny, Kianian Shahryar F, Zhang Chi, Holding David
Abstract excerpt
To better understand maize endosperm filling and maturation, we used γ-irradiation of the B73 maize reference line to generate mutants with opaque endosperm and reduced kernel fill phenotypes, and created a population of 1788 lines including 39 Mo17 × F2s showing stable, segregating, and viable kernel phenotypes. For molecular characterization of the mutants, we developed a novel functional genomics platform that...
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