Article
Functional characterization of SlitPBP3 in Spodoptera litura by CRISPR/Cas9 mediated genome editing.
Insect biochemistry and molecular biology - 1 Aug 2016
Zhu Guan-Heng, Xu Jun, Cui Zhen, Dong Xiao-Tong, Ye Zhan-Feng, Niu Dong-Juan, Huang Yong-Ping, Dong Shuang-Lin
Abstract excerpt
Functional gene analysis by using genome editing techniques is limited only in few model insects. Here, we reported an efficient and heritable gene mutagenesis analysis in an important lepidopteran pest, Spodoptera litura, using the CRISPR/Cas9 system. By using this system, we successfully obtained the homozygous S. litura strain by targeting the pheromone binding protein 3 gene (SlitPBP3), which allowed us to...
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