Structural heterogeneity and precision of implications drawn from cryo-electron microscopy structures: SARS-CoV-2 spike-protein mutations as a test case.

Abstract excerpt

Protein structures may be used to draw functional implications at the residue level, but how sensitive are these implications to the exact structure used? Calculation of the effects of SARS-CoV-2 S-protein mutations based on experimental cryo-electron microscopy structures have been abundant during the pandemic. To understand the precision of such estimates, we studied three distinct methods to estimate stability...

Topics

• Humans
• Spike Glycoprotein, Coronavirus
• Cryoelectron Microscopy
• SARS-CoV-2
• COVID-19
• Models, Molecular
• Mutation
• Proteins