Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Thermostability Engineering of a Chitin-Utilizing GlcNAc Deacetylase from Acinetobacter baumannii via Distal Mutations.

Journal of agricultural and food chemistry - 22 Jul 2026

Sun Ze-Rui, Xia Yu-Xin, Li Yi-Ran, Liu Li, Voglmeir Josef

Abstract excerpt

The deacetylation of N-acetylglucosamine (GlcNAc) to glucosamine (GlcN) is a key step in enzymatic chitin valorization. We identified a novel GlcNAc deacetylase (AbNGD) from Acinetobacter baumannii with high substrate specificity and catalytic activity but poor thermal stability (half-life of 5.5 h at 37 °C). Using the FireProt platform, we generated a combinatorial mutant, AbNGD-M3 (T93P-C153T-A244M). Its...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Acetylglucosamine
Share to Acinetobacter baumannii

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Amidohydrolases

Share to Chitin

Share to Enzyme Stability

Share to Hot Temperature

Share to Molecular Dynamics Simulation

Share to Protein Engineering

Source

Identifiers

PMID: 42443049
DOI: 10.1021/acs.jafc.6c07357

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Thermostability Engineering of a Chitin-Utilizing GlcNAc Deacetylase from Acinetobacter baumannii via Distal Mutations.PMID 42443049Read ↗

Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.Semantic proximity Read ↗

Inverse relationship between chitobiase and transglycosylation activities of chitinase-D from Serratia proteamaculans revealed by mutational and biophysical analyses.Semantic proximity Read ↗

Free energy-guided Q302P mutation in GH1 β-glucosidase from Aspergillus fumigatus improves enzyme function via catalytic tunnel perturbation.Semantic proximity Read ↗

Selection and mutational analyses of the substrate interacting residues of a chitinase from Enterobacter cloacae subsp. cloacae (EcChi2) to improve transglycosylation.Semantic proximity Read ↗

Rational Engineering of a β-Glucosidase (H0HC94) from Glycosyl Hydrolase Family I (GH1) to Improve Catalytic Performance on Cellobiose.Semantic proximity Read ↗

Active-site mutations improved the transglycosylation activity of Stenotrophomonas maltophilia chitinase A.Semantic proximity Read ↗

Two-step computational redesign of Bacillus subtilis cellulase and β-glucanase for enhanced thermostability and activity.Semantic proximity Read ↗

Targeting a critical step in fungal hexosamine biosynthesisSemantic proximity Read ↗

Engineering Bacterial Chitinases for Industrial Application: From Protein Engineering to Bacterial Strains Mutation! A Comprehensive Review of Physical, Molecular, and Computational Approaches.Semantic proximity Read ↗

Biochemical analysis and structure determination of bacterial acetyltransferases responsible for the biosynthesis of UDP-N,N'-diacetylbacillosamine.Semantic proximity Read ↗

Aromatic residues in the catalytic center of chitinase A from Serratia marcescens affect processivity, enzyme activity, and biomass converting efficiency.Semantic proximity Read ↗

Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formationSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.