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Article

Analysis of the raw starch-binding domain by mutation of a glucoamylase from Aspergillus awamori var. kawachi expressed in Saccharomyces cerevisiae.

Applied and environmental microbiology - 1 Nov 1994

Goto M, Semimaru T, Furukawa K, Hayashida S

Abstract excerpt

Carboxy-terminal deletions were introduced into the raw starch-binding domain (A-515 to R-615) encoded by the gene for glucoamylase I (GAI) from Aspergillus awamori var. kawachi. Genes coding for proteins designated GA596 (A-1 to E-596), GA570 (A-1 to A-570), and GA559 (A-1 to N-559) were constructed and resulted in truncated proteins. All of the mutant genes were expressed heterologously in Saccharomyces...

Topics

Amino Acid Sequence
Aspergillus
Base Sequence
Gene Expression Regulation, Enzymologic
Genetic Vectors
Glucan 1,4-alpha-Glucosidase
Molecular Sequence Data
Mutation
Saccharomyces cerevisiae
Starch

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Identifiers

PMID: 7993082
DOI: 10.1128/aem.60.11.3926-3930.1994
PMCID: PMC201917

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7993082-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Analysis of the raw starch-binding domain by mutation of a glucoamylase from Aspergillus awamori var. kawachi expressed in Saccharomyces cerevisiae.PMID 7993082Read ↗

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