Article
Influence of different substrates on the production of a mutant thermostable glucoamylase in submerged fermentation.
Applied biochemistry and biotechnology - 1 Jan 2011
Pavezzi Fabiana Carina, Carneiro Andréia A Jacomassi, Bocchini-Martins Daniela Alonso, Alves-Prado Heloiza Ferreira, Ferreira Henrique, Martins Paula M, Gomes Eleni, da Silva Roberto
Abstract excerpt
Three mutations, Ser54→Pro, Thr314→Ala, and His415→Tyr, were identified in Aspergillus awamori glucoamylase gene expressed by Saccharomyces cerevisiae. The mutant glucoamylase (GA) was substantially more thermostable than a wild-type GA at 70 °C, with a 3.0 KJ mol(-1) increase in the free energy of thermo-inactivation. The effect of starch from different botanical sources on the production of this GA was measured...
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