Article
Improvement of enzymatic properties of Rhizopus oryzae α-amylase by site-saturation mutagenesis of histidine 286.
Enzyme and microbial technology - 1 Oct 2018
Li Song, Yang Qian, Tang Bin, Chen Ana
Abstract excerpt
Optimal pH and ideal functioning temperature for fungal α-amylase can greatly contribute to improving enzyme efficiency in maltose-forming ability. This work aimed to improve the enzymatic properties of Rhizopus oryzae α-amylase by site-saturation mutagenesis of histidine 286. The biochemical properties of selected mutant enzymes were modified to increase their enzymatic efficiencies compared to their wild-type...
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