Article
Protein Conformational Space at the Edge of Allostery: Turning a Nonallosteric Malate Dehydrogenase into an "Allosterized" Enzyme Using Evolution-Guided Punctual Mutations.
Molecular biology and evolution - 1 Sept 2022
Iorio Antonio, Brochier-Armanet Céline, Mas Caroline, Sterpone Fabio, Madern Dominique
Abstract excerpt
We unveil the intimate relationship between protein dynamics and allostery by following the trajectories of model proteins in their conformational and sequence spaces. Starting from a nonallosteric hyperthermophilic malate dehydrogenase, we have tracked the role of protein dynamics in the evolution of the allosteric capacity. Based on a large phylogenetic analysis of the malate (MalDH) and lactate dehydrogenase...
Topics
- Allosteric Regulation
- Amino Acids
- L-Lactate Dehydrogenase
- Malate Dehydrogenase
- Malates
- Mutation
- Phylogeny