Article
A domesticated transposon mediates the effects of a single-nucleotide polymorphism responsible for enhanced muscle growth.
EMBO reports - 1 Apr 2010
Butter Falk, Kappei Dennis, Buchholz Frank, Vermeulen Michiel, Mann Matthias
Abstract excerpt
Single-nucleotide polymorphisms (SNPs) in the regulatory regions of the genome can have a profound impact on phenotype. The G3072A polymorphism in intron 3 of insulin-like growth factor 2 (IGF2) is implicated in higher muscle content and reduced fat in European pigs and is bound by a putative repressor. Here, we identify this repressor--which we call muscle growth regulator (MGR)--by using a DNA protein...
Topics
- Animals
- Blotting, Western
- Cell Line
- Chromatin Immunoprecipitation
- DNA Transposable Elements
- Electrophoretic Mobility Shift Assay
- Humans
- Insulin-Like Growth Factor II
- Introns
- Mice
- Muscles
- Polymorphism, Single Nucleotide
- Promoter Regions, Genetic
- Protein Binding